Solution Informationhelp
Enzyme: Cyclin-dependent kinase 7
inhibitor: BDBM32352
substrate: n/a
 
Solution Type: Aqueous
pH at Preparation: n/a
Temp. Prep.: n/a
Comments: Protocol CDK7 (CAK complex) counterscreening assay protocol for identified PKD inhibitors (384-well HTS) Reagents 1X kinase reaction buffer 10 mM Tris-HCl, pH 7.2 10 mM MgCl2 0.05% NaN3 0.1% Tween-20 mM DTT Substrate/ATP (3X concentration) 1500 nM substrate peptide 75 uM ATP in 1X kinase reaction buffer Max control (3X concentration) 3% DMSO in 1X kinase reaction buffer Min control (3X concentration) 150 uM H-89 in 1X kinase reaction buffer Compounds (3X concentration) 2 uL of 1 mM compounds solubilized in 1X kinase reaction buffer starting at 300 uM (3X concetration) and subjected to a 2-fold serial dilution Enzyme (3X concentration) 0.1 mUnits/uL in 1X kinase reaction buffer Binding reagent 1:400 dilution binding reagent in 70% 1X binding buffer A and 30% 1X binding buffer B with 1:400 terbium Assay Procedure (1)Prepare reagents as described above (2)Add 2 uL of a substrate peptide and ATP mixture to appropriate wells. (3)Add 2 uL of test compound dilution or controls to
 
 
If you find an error in this entry please send us an E-mail